Left ventricular midwall mechanics at 24 weeks' gestation in high-risk normotensive pregnant women: Relationship to placenta-related complications of pregnancy

Most studies during pregnancy have assessed maternal left ventricular (LV) function by load-dependent indices, assessing only chamber function. The aim of this study was to assess afterload-adjusted LV myocardial and chamber systolic function at 24 weeks' gestation and 6 months postpartum in high-risk normotensive pregnant women

Generation of ribosome imprinted polymers for sensitive detection of translational responses

Whilst the profiling of the transcriptome and proteome even of single-cells becomes feasible, the analysis of the translatome, which refers to all messenger RNAs (mRNAs) engaged with ribosomes for protein synthesis, is still an elaborate procedure requiring millions of cells. Herein, we report the generation and use of “smart materials”, namely molecularly imprinted polymers (MIPs) to facilitate the isolation of ribosomes and translated mRNAs from merely 1,000 cells. In particular, we show that a hydrogel-based ribosome imprinted polymer could recover ribosomes and associated mRNAs from human, simian and mice cellular extracts, but did not selectively enrich yeast ribosomes, thereby demonstrating selectivity. Furthermore, ribosome imprinted polymers enabled the sensitive measurement of an mRNA translational regulatory event, requiring 1,000-fold less cells than current methodologies. These results provide first evidence for the suitability of MIPs to selectively recover ribonucleoprotein complexes such as ribosomes, founding a novel means for sensitive detection of gene regulation

Catalytic and selective synthesis of lactones and bis-lactones by palladium acetate/1,4-bis(diphenylphosphino) butane system under syngas conditions

The combination of palladium acetate and 1,4-bis(diphenylphosphino)butane (dppb) proved once again to form an effective catalytic system for the selective cyclocarbonylation of 2-allylphenols and bis-2-allylphenols. The reactions afforded five-, six- and seven-membered ring lactones and bis-lactones. The seven-membered ring lactones were predominant in most reactions

A Novel Rh(I)-PCy3-CO-HCOOH Catalytic System fot the Regioselective Conversion of Alkenes to Aldehydes

Rhodium carbonyl and tricyclohexylphosphine, in the presence of CO and HCOOH, is an efficient catalytic system for the regioselective conversion of alkenes into branched and linear aldehydes. This process shows excellent functional group tolerance